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penta-histm antibody  (Qiagen)


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    Structured Review

    Qiagen penta-histm antibody
    Penta Histm Antibody, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/penta+histm+antibody/penta+histm+antibody/us11292819-737-14-16
    Average 90 stars, based on 1 article reviews
    penta-histm antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Western Blot:

    Article Title: Methods for protein screening
    Article Snippet: .. Harlow, E. and Lane, D. Cold Spring Harbor Laboratory Press 1988 New York, USA and Qiagen Western and Dot Blotting Protocols September 1997), detecting the translated protein with Penta HisTM antibody (0.1 μg/ml) (Qiagen Ltd) or anti-FLAG M2 antibody (1.4 μg/ml) (Kodak Scientific Imaging Systems Ltd, New York, USA), followed by anti-mouse IgG HRP conjugate (0.5 μg/ml) (Sigma, Poole, UK.). ..

    Article Title: WbpO, a UDP-N-acetyl-d-galactosamine Dehydrogenase from Pseudomonas aeruginosa Serotype O6
    Article Snippet: .. Western immunoblotting following SDS-polyacrylamide gel electrophoresis was performed according to Burnette (26) using Penta-HisTM Antibody (Qiagen, Mississauga, Ontario, Canada) diluted to 1:1,000 in 3% bovine serum albumin in Tris-buffered saline as the first antibody. .. CD Spectrometry—CD of WbpO protein was measured with a JASCO J-600 spectropolarimeter (Japan Spectroscopic Co. Ltd., Tokyo, Japan) at 20 °C.

    Article Title: Engineering pathogen resistance in plants
    Article Snippet: In Vitro Sulfation of RaxX by his-RaxST RaxST was expressed as a full length fusion protein with an N-terminal His-tag in BL21(DE3) cells and purified using Ni-NTA agarose beads (QIAGEN). .. His-RaxST expression was confirmed by SDS-PAGE with Coomassie-blue staining and western blot analysis with Penta-HiSTM antibody (QIAGEN) (FIGS. 18A-18B). ..

    Imaging:

    Article Title: Methods for protein screening
    Article Snippet: .. Harlow, E. and Lane, D. Cold Spring Harbor Laboratory Press 1988 New York, USA and Qiagen Western and Dot Blotting Protocols September 1997), detecting the translated protein with Penta HisTM antibody (0.1 μg/ml) (Qiagen Ltd) or anti-FLAG M2 antibody (1.4 μg/ml) (Kodak Scientific Imaging Systems Ltd, New York, USA), followed by anti-mouse IgG HRP conjugate (0.5 μg/ml) (Sigma, Poole, UK.). ..

    Generated:

    Article Title: The CARF Protein MM_0565 Affects Transcription of the Casposon-Encoded cas1-solo Gene in Methanosarcina mazei Gö1
    Article Snippet: Cell extracts (750 μg) or purified His 6 -MM_0565 protein were separated on 12% SDS-PAGE and transferred to nitrocellulose membranes (BioTrace ® NT, Pall Life Sciences, Port Washington, NY, USA) [ ]. .. Membranes were exposed to specific Penta-HisTM antibody (Qiagen, Cat. No. 34660, Hilden, Germany) directed against the His-tag or with an antiserum generated against MM_0565. .. Protein bands were detected with secondary antibodies directed against mouse IgG or rabbit IgG coupled to horseradish peroxidase (Bio-Rad Laboratories, Hercules, CA, USA) and visualized using the ECLTM plus system (GE Healthcare, Chicago, IL, USA) with a fluoro-imager (DianaIII, Raytest, Straubenhardt, Germany).

    Nucleic Acid Electrophoresis:

    Article Title: WbpO, a UDP-N-acetyl-d-galactosamine Dehydrogenase from Pseudomonas aeruginosa Serotype O6
    Article Snippet: .. Western immunoblotting following SDS-polyacrylamide gel electrophoresis was performed according to Burnette (26) using Penta-HisTM Antibody (Qiagen, Mississauga, Ontario, Canada) diluted to 1:1,000 in 3% bovine serum albumin in Tris-buffered saline as the first antibody. .. CD Spectrometry—CD of WbpO protein was measured with a JASCO J-600 spectropolarimeter (Japan Spectroscopic Co. Ltd., Tokyo, Japan) at 20 °C.

    Saline:

    Article Title: WbpO, a UDP-N-acetyl-d-galactosamine Dehydrogenase from Pseudomonas aeruginosa Serotype O6
    Article Snippet: .. Western immunoblotting following SDS-polyacrylamide gel electrophoresis was performed according to Burnette (26) using Penta-HisTM Antibody (Qiagen, Mississauga, Ontario, Canada) diluted to 1:1,000 in 3% bovine serum albumin in Tris-buffered saline as the first antibody. .. CD Spectrometry—CD of WbpO protein was measured with a JASCO J-600 spectropolarimeter (Japan Spectroscopic Co. Ltd., Tokyo, Japan) at 20 °C.

    Expressing:

    Article Title: Engineering pathogen resistance in plants
    Article Snippet: In Vitro Sulfation of RaxX by his-RaxST RaxST was expressed as a full length fusion protein with an N-terminal His-tag in BL21(DE3) cells and purified using Ni-NTA agarose beads (QIAGEN). .. His-RaxST expression was confirmed by SDS-PAGE with Coomassie-blue staining and western blot analysis with Penta-HiSTM antibody (QIAGEN) (FIGS. 18A-18B). ..

    SDS Page:

    Article Title: Engineering pathogen resistance in plants
    Article Snippet: In Vitro Sulfation of RaxX by his-RaxST RaxST was expressed as a full length fusion protein with an N-terminal His-tag in BL21(DE3) cells and purified using Ni-NTA agarose beads (QIAGEN). .. His-RaxST expression was confirmed by SDS-PAGE with Coomassie-blue staining and western blot analysis with Penta-HiSTM antibody (QIAGEN) (FIGS. 18A-18B). ..

    Staining:

    Article Title: Engineering pathogen resistance in plants
    Article Snippet: In Vitro Sulfation of RaxX by his-RaxST RaxST was expressed as a full length fusion protein with an N-terminal His-tag in BL21(DE3) cells and purified using Ni-NTA agarose beads (QIAGEN). .. His-RaxST expression was confirmed by SDS-PAGE with Coomassie-blue staining and western blot analysis with Penta-HiSTM antibody (QIAGEN) (FIGS. 18A-18B). ..



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